Red blood cell antibody screening and identification in thalassemia major patients
Main Article Content
Keywords
alloimmunization, regular blood transfusion, screening antibody, thalassemia major
Abstract
Introduction: Blood transfusion is a medical intervention for thalassemia major to maintain hemoglobin levels between 9-10,5 g/dl. Since it is practically given continuously for life, unexpected complications might occur. Alloimmunization might still happen during transfusion despite being tested for ABO blood group and Rhesus D type and compatibility. Hemolytic reaction as a complication of this transfusion practice would eventually make the patient obtain more frequent blood transfusions. This study aims to examine antibody profiles in thalassemia major patients receiving a long-term regular blood transfusion.
Methods: This was a cross sectional study. Venous blood was drawn in a 10 ml syringe from each patient and then divided into a test tube with anticoagulant and another tube without anticoagulant to prepare plasma and serum. The ABO blood group, Rhesus, and Kell were tested using a tube test, the continued Direct Coombs Test (DCT), and the Rubin test for eluate. Antibody screening and identification using a cell panel were performed for positive results.
Results: 15 (14,29%) of 105 patients were positive for DCT, eluate, screening and antibody identification. This positive result was caused by probable drug-induced (33,36%), warm type AIHA (6,66%), Lea, Leb and Jkb (6,66%).
Conclusions: Alloantibody was rarely found in thalassemia major patients receiving long-term regular blood transfusions.
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